DreamTaq™ DNA Polymerase generates PCR products with 3′-dA overhangs. The enzyme is . software at or follow the general . In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this. Thermo Scientific™ DreamTaq DNA Polymerase. Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this.

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Fermentas DreamTaq DNA Polymerase, 5 x 500U

For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq?

Data for box pcr reaction mix gathered from related PubMed articles. The dyes in fedmentas master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Annealing for 30 seconds is normally sufficient. Gently vortex the samples and spin down. Thermo Fisher dreamtaq buffer Bioz Stars score: For degenerate primers and primers used for long PCR we recommend higher primer concentrations in the range of 0.


Opinions on Fermentas DreamTaq Green PCR Master Mix? – PCR Reagents and Equipments

For higher template amounts, cycles are sufficient. Ribonuclease Assay Less than 0.

Avoid self-complementary primer regions, complementarities between the primers and direct primer repeats to prevent hairpin dreammtaq and primer dimerization.

Dreamtsq a thin-walled PCR tube on ice and add the following components for each 50? Higher amounts of template increase the risk of generation of non-specific PCR products. This product is licensed under U. Extension The optimal extension temperature for DreamTaq? Lower amounts of template reduce the accuracy of the amplification. Use reagent containers dedicated for PCR.

All routine DNA purification methods can be used to prepare the template e. Analyses on plant derived writing supports and non-invasive DNA sampling Due to fragmentation, only short amplicons were tested for historic samples.


Ordering Information Component DreamTaq? Data for dreamtaq buffer gathered from related PubMed articles. Wear fresh gloves for DNA purification and reaction set up.

Ideally, C and G nucleotides should be distributed uniformly along fegmentas primer. FPa core-genome gene highly conserved in F. Green buffer is a proprietary formulation optimized for robust performance in PCR.

When using a thermal cycler that does not contain a heated lid, overlay the reaction mixture with 25? If the primer contains more than 25 nucleotides we recommend using specialized computer programs e. All cDNA samples were run in triplicates and water was used as a fermenttas control. PCR primers are generally nucleotides long.


Estimation of primer melting temperature For primers containing less than 25 nucleotides, the approx. In certain dreamtaa use of this product is covered by patents. The reaction mixture for PCR consisted of: Gently vortex and briefly centrifuge DreamTaq?

PCR conditions were as given in Additional file.

Therefore, care must be taken to avoid contamination with other templates and amplicons that may be present in the laboratory environment. General recommendations to lower the risk of contamination are as follows: Omitting the primers sequences, the amplified fragment was nucleotides long.

Primer design and quantitative real-time PCR This product is licensed under one or more U.